The pitches "middle C," "G above middle C," and "F below middle C" are equivalent to the following scientific pitch notations as follows -
- Middle C is C4
- G above middle C is G4, and
- F below middle C is F3
- First, the guitar has 6 strings that are tuned differently in open and 18-24 frets.
- Each fret advances the note placed before it by a semitone
A particular pitch (frequency) can occur on more than one string. From the above, it is clear that a pitch must be playable in at least 3 different string-fret combination to make up the fabled 144.This is true and the locations of the notes mentioned above on a standard 6 string, 24 fret guitar tuned to standard tuning are as follows:
| Middle C | F above C4 | G below C4 |
| S: 6; F:20 | S: 6; F:11 | S: 1; F: 3 |
| S: 5; F:15 | S: 5; F: 8 | S: 2; F: 8 |
| S: 4; F:10 | S: 4; F: 3 | S: 3; F:12 |
| S: 3; F: 5 | x | S: 4; F:17 |
| S: 2; F: 1 | x | S: 5; F:22 |
N.B. S means "sting number" and F means "fret number." Calculations were done, taking S2;F:1 as middle C and going above and to the right to arrive at middle C every time, and similarly for the other two.
There is one thing different though - as one changes the strings, the timbre of the note (not the pitch /frequency) changes.

